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anti par mar  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti par mar
    Anti Par Mar, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mar+par/bio_rxiv__64898__2026__03__31__715305-239-11-13?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    anti par mar - by Bioz Stars, 2026-08
    86/100 stars

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    anti mar par e6f6a  (Cell Signaling Technology Inc)
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    ( A ) HeLa cells were treated with 10 µM deubiquitinase inhibitor (DUBi) PR-619 for 30 min, followed by fixation in methanol and staining using a PAR/MAR antibody from Cell Signaling Technology <t>(E6F6A).</t> The mounted cells were imaged using a confocal microscope. ( B ) As in ( A ), with fixation of the cells in glyoxal. ( C , D ) As in ( A , B ), using a mono(ADP-ribose)-specific antibody from BioRad (HCA354). Scale bars represent 20 µm. To enhance the visibility of foci, grayscale images of the MAR/PAR or MAR signals were inverted and shown on the left of each panel. On the right of each panel, merged images displaying DNA in blue and MAR/PAR or MAR signal in green.
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    secondary antibodies primary antibodies source identifier mar par cell signaling  (Cell Signaling Technology Inc)
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    ( A ) HeLa cells were treated with 10 µM deubiquitinase inhibitor (DUBi) PR-619 for 30 min, followed by fixation in methanol and staining using a PAR/MAR antibody from Cell Signaling Technology <t>(E6F6A).</t> The mounted cells were imaged using a confocal microscope. ( B ) As in ( A ), with fixation of the cells in glyoxal. ( C , D ) As in ( A , B ), using a mono(ADP-ribose)-specific antibody from BioRad (HCA354). Scale bars represent 20 µm. To enhance the visibility of foci, grayscale images of the MAR/PAR or MAR signals were inverted and shown on the left of each panel. On the right of each panel, merged images displaying DNA in blue and MAR/PAR or MAR signal in green.
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    ( A ) HeLa cells were treated with 10 µM deubiquitinase inhibitor (DUBi) PR-619 for 30 min, followed by fixation in methanol and staining using a PAR/MAR antibody from Cell Signaling Technology <t>(E6F6A).</t> The mounted cells were imaged using a confocal microscope. ( B ) As in ( A ), with fixation of the cells in glyoxal. ( C , D ) As in ( A , B ), using a mono(ADP-ribose)-specific antibody from BioRad (HCA354). Scale bars represent 20 µm. To enhance the visibility of foci, grayscale images of the MAR/PAR or MAR signals were inverted and shown on the left of each panel. On the right of each panel, merged images displaying DNA in blue and MAR/PAR or MAR signal in green.
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    Image Search Results


    ( A ) HeLa cells were treated with 10 µM deubiquitinase inhibitor (DUBi) PR-619 for 30 min, followed by fixation in methanol and staining using a PAR/MAR antibody from Cell Signaling Technology (E6F6A). The mounted cells were imaged using a confocal microscope. ( B ) As in ( A ), with fixation of the cells in glyoxal. ( C , D ) As in ( A , B ), using a mono(ADP-ribose)-specific antibody from BioRad (HCA354). Scale bars represent 20 µm. To enhance the visibility of foci, grayscale images of the MAR/PAR or MAR signals were inverted and shown on the left of each panel. On the right of each panel, merged images displaying DNA in blue and MAR/PAR or MAR signal in green.

    Journal: The EMBO Journal

    Article Title: PARP7 is a proteotoxic stress sensor that labels proteins for degradation

    doi: 10.1038/s44318-025-00545-7

    Figure Lengend Snippet: ( A ) HeLa cells were treated with 10 µM deubiquitinase inhibitor (DUBi) PR-619 for 30 min, followed by fixation in methanol and staining using a PAR/MAR antibody from Cell Signaling Technology (E6F6A). The mounted cells were imaged using a confocal microscope. ( B ) As in ( A ), with fixation of the cells in glyoxal. ( C , D ) As in ( A , B ), using a mono(ADP-ribose)-specific antibody from BioRad (HCA354). Scale bars represent 20 µm. To enhance the visibility of foci, grayscale images of the MAR/PAR or MAR signals were inverted and shown on the left of each panel. On the right of each panel, merged images displaying DNA in blue and MAR/PAR or MAR signal in green.

    Article Snippet: Antibodies used: rabbit anti-ADPr (HCA354, BioRad), rabbit anti-MAR/PAR E6F6A (CST, lot 5; Cat 83732S), mouse anti-ubiquitin P4D1 (Santa Cruz, sc-8017, Lot D0123), mouse anti-mono and polyubiquitinated conjugates FK2 (BIOZOL, Cat UBI-68-0122-100), mouse anti-Tubulin-alpha (Santa Cruz, sc-5286, Lot L1522), mouse anti-p62 (Santa Cruz, sc-28359, Lot H2621), rabbit anti-GFP (Chromotek, Cat pabg1, Lot 70828032AB), mouse anti-HA (Covance, Cat MMS-101R), mouse anti-LC3B (G-9) (Santa Cruz, Cat sc-376404, Lot G2523), Rabbit anti-LC3B (CST, Cat 2775S, Lot 14), mouse anti-GAPDH (Santa Cruz, Cat sc-32233, Lot J2523) and rabbit anti-c-FOS (66590-1-Ig, Proteintech).

    Techniques: Staining, Microscopy